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KMID : 0360320010330050385
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Journal of Korean Cancer Research Association
2001 Volume.33 No. 5 p.385 ~ p.391
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Effects of Polyamines on TNFα-or Tamoxifen-induced Apoptosis in Human Breast Cancer Cells
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김지영/Ji Young Kim
김기영/이경희/홍기환/김병기/Ki Young Kim/Kung Hee Lee/Ki Whan Hong/Byeong Gee Kim
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Abstract
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PURPOSE:
To investigate the effects of polyamines on tumor necrosis factor alpha (TNFalpha)-or tamoxifen (TAM)-induced apoptosis in estrogen receptor (ER)-positive MCF- 7 and ER-negative MDA-MB-231 human breast cancer cells.
MATERIALS AND METHODS:
Cell viability was assessed by using MTT assay. Reactive oxygen species (ROS) generation was measured using 2', 7'-dichlorofluorescin diacetste (DCFDA) by fluorescence plate reader. DNA fragmentation was assessed by 1.5% agarose gel electrophoresis.
RESULTS:
TNFalpah and TAM showed significant dose- and time- dependent inhibitory effects on the growth of MCF-7 human cells. However, the growth of MDA-MB-231 cells were not inhibited by TNFalpha or TAM treatment. The generation of ROS was increased in dose-and time-dependent manner by TNFalpha treatment in MCF-7 cells. Polyamines, especially spermine suppressed TNFalpha-induced ROS generation in MCF-7 cells. Antioxidant effects of polyamines were also demonstrated by DNA fragmentation, cell morphology as well as ROS generation assay. Polyamines also blocked TAM-induced cell death in MCF-7 cell. However, MDA-MB-231 cells showed resistance to the cytotoxic effects of TNFalpha or TAM.
CONCLUSION:
These results suggest that polyamines may prevent TNFalpha or TAM-induced apoptosis in MCF-7 human breast cancer cells.
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KEYWORD
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Polyamines, Apoptosis, TNFα, Tamoxifen,
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